ChIP Assay for Epigenetic Profiling

ChIP assays were performed as described [28 (link)], using antibodies: H3K4me2 (07-030, Millipore, Billerica, MA, http://www.emdmillipore.com/), LSD1 (sc-49291, Santa Cruz Biotechnology), Pit-1 (sc-442, Santa Cruz Biotechnology), and receptor-interaction protein 140 (RIP140; ab42126, Abcam). Primers used for ChIP assay are shown in Supporting Information Table S1.

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Wu C.Y., Persaud S.D, & Wei L.N. (2015). Retinoic Acid Induces Ubiquitination-Resistant RIP140/LSD1 Complex to Fine-Tune Pax6 Gene in Neuronal Differentiation. Stem cells (Dayton, Ohio), 34(1), 114-123.

Publication 2015

H3K4me2 Pit-1 ab42126

Antibodies Chip assay Lsd1 Primers Protein Rip140

Corresponding Organization : University of Minnesota Medical Center

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Variable analysis

independent variables

Antibodies used for ChIP assays: H3K4me2, LSD1, Pit-1, and receptor-interaction protein 140 (RIP140)

dependent variables

Outcomes measured in the ChIP assays

control variables

Experimental protocol for ChIP assays as described in reference [28]
Primers used for ChIP assay as shown in Supporting Information Table S1

controls

Positive controls: Not specified
Negative controls: Not specified

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