Imaging Septin Dynamics on Curved Membranes

Images of fluorescent-tagged septins adsorbed onto curved supported bilayers on microspheres were acquired using a spinning disc (Yokogawa W1) confocal microscope (Nikon Ti-82 stage) using a 100× Plan Apo 1.49 NA oil lens and a Prime 95B CMOS camera (Photometrics). Images were analyzed using Imaris 8.1.2 software (Bitplane AG) as previously described (Bridges et al., 2016 (link); Cannon et al., 2019 (link)).

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Cannon K.S., Vargas-Muniz J.M., Billington N., Seim I., Ekena J., Sellers J.R, & Gladfelter A.S. (2023). A gene duplication of a septin reveals a developmentally regulated filament length control mechanism. The Journal of Cell Biology, 222(3), e202204063.

Publication 2023

Ti-82 stage Imaris 8.1.2 software

Cmos Confocal microscope Lens Microspheres Septins

Corresponding Organization : Marine Biological Laboratory

Other organizations : Southern Illinois University Carbondale, National Heart Lung and Blood Institute, National Institutes of Health

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Variable analysis

independent variables

Images of fluorescent-tagged septins adsorbed onto curved supported bilayers on microspheres

dependent variables

Measured outcomes

control variables

Spinning disc (Yokogawa W1) confocal microscope (Nikon Ti-82 stage) using a 100× Plan Apo 1.49 NA oil lens and a Prime 95B CMOS camera (Photometrics)
Imaris 8.1.2 software (Bitplane AG) for image analysis

controls

No specific positive or negative controls were mentioned in the input.

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