Measuring Cell Proliferation and Viability

The Cell-Light 5-ethynyl-2-deoxyuridine (EdU) Apollo 567 Kits (RiboBio, Guangzhou, China) were used to detect cell proliferation. Cell proliferation rate = (EdU-positive cells)/(total cells) × 100% [54 (link)]. The 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) (Sigma, St. Louis, MO, USA) and CCK-8 detection kits (Beyotime, Shanghai, China) were adopted to determine cell viability [55 (link)–57 (link)].

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Duan S.F., Zhang M.M., Dong Q., Yang B., Liu W., Zhang X., Yu H.L., Zhang S.H., Khan N.H., Wu D.D., Zhang X.J, & Cen J. (2023). A Water-Soluble Hydrogen Sulfide Donor Suppresses the Growth of Hepatocellular Carcinoma via Inhibiting the AKT/GSK-3β/β-Catenin and TGF-β/Smad2/3 Signaling Pathways. Journal of Oncology, 2023, 8456852.

Publication 2023

Cell-Light 5- 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2-H-tetrazolium bromide (MTT) CCK-8 detection kits

Bromide Cck 8 Cell proliferation Cell viability Cells Deoxyuridine Diphenyl Light Tetrazolium

Corresponding Organization : Zhengzhou University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Cell proliferation rate
Cell viability

control variables

None explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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