Pharmacological Modulation of Duchenne Muscular Dystrophy
Starting at 2 wk of age, mdx mice were treated daily by intraperitoneal injection for 4 wk with 125 mg/kg body weight Coomassie Brilliant Blue G 250 (CBB) (Sigma-Aldrich) or for 4 wk with 8.4 mg/kg body weight oxidized ATP (ox-ATP) (Tocris Bioscience) or for 2 wk with 50 mg/kg body weight A-438079 (Tocris Bioscience). Dosage was based on previous studies [9 (link),23 (link),24 (link)]. Age-matched control mice received the same volume of sterile saline (for CBB and ox-ATP) or saline with 20% v/v DMSO solution (for A-438079).
Sinadinos A., Young C.N., Al-Khalidi R., Teti A., Kalinski P., Mohamad S., Floriot L., Henry T., Tozzi G., Jiang T., Wurtz O., Lefebvre A., Shugay M., Tong J., Vaudry D., Arkle S., doRego J.C, & Górecki D.C. (2015). P2RX7 Purinoceptor: A Therapeutic Target for Ameliorating the Symptoms of Duchenne Muscular Dystrophy. PLoS Medicine, 12(10), e1001888.
Body weight Coomassie brilliant blue g 250 Dmso Intraperitoneal injection Mdx mice Mice Oxidized atpSaline solution Sterile
Corresponding Organization : University of Portsmouth
Other organizations :
University of L'Aquila, Pittsburg State University, University of Pittsburgh, Université de Rouen Normandie, Inserm, Institute of Bioorganic Chemistry, Pirogov Russian National Research Medical University, Centre National de la Recherche Scientifique
Treatment with 125 mg/kg body weight Coomassie Brilliant Blue G 250 (CBB) for 4 weeks
Treatment with 8.4 mg/kg body weight oxidized ATP (ox-ATP) for 4 weeks
Treatment with 50 mg/kg body weight A-438079 for 2 weeks
dependent variables
Not explicitly mentioned
control variables
Age-matched control mice received the same volume of sterile saline (for CBB and ox-ATP)
Age-matched control mice received the same volume of saline with 20% v/v DMSO solution (for A-438079)
controls
Positive control: None specified
Negative control: Age-matched control mice received the same volume of sterile saline (for CBB and ox-ATP) or saline with 20% v/v DMSO solution (for A-438079)
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