Western Blotting for Protein Analysis

Western blotting assays were performed as previously described [16 (link)]. The following antibodies were used in the analysis: anti-ADAM15 (sc-365752), anti-CD151 (sc-271216) (Santa Cruz, CA, USA), anti-Integrin αV (ab179475), anti-Integrin α3 (ab242196), anti-Integrin α6 (ab20142), and anti-Integrin β1 (ab52971) antibodies (Abcam, London, UK), followed by the p-Her2 (Sigma-Aldrich; Merck KGaA, SAB4300061), anti-p-EGFR (#3777), anti-EGFR (#4267), anti-Her2 (#4290), anti-p-FAK (#8556), anti-FAK (#13009), anti-p-AKT (#4060), anti-AKT (#4691), anti-p-ERK (#4370), anti-ERK (#4695), anti-CyclinD1 (#2978), anti-MMP9 (#13667) (Cell Signaling Technology Danvers, MA, USA). Anti-β-actin (CW0096M) and anti-mouse (CW0102) or anti-rabbit (CW0103) secondary antibodies were purchased from Cowin (China).