HIV-1 Diagnosis and Viral Load Quantification

The HIV-1 diagnosis was made according to Alere Determine HIV-1/2 Ag/Ab Combo test in Zambia and Tanzania HIV Rapid Test Algorithm. The HIV-1 serology results were verified using HIV-1–2.0 First Response kit (Premier Medical Corporation Ltd., Mumbai, India). To quantify HIV-1 plasma viral load, the viral RNA was extracted from plasma following the QIAamp viral RNA extraction protocol (Qiagen, Hilden, Germany) and measured using the RNA Ultra-Sense One-Step quantitative real-time PCR (qPCR) system (Applied Biosystems, Foster City, CA, USA) as previously published [33 (link)].

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Mortazavi Y., Lidenge S.J., Tran T., West J.T., Wood C, & Tso F.Y. (2020). The Kaposi’s Sarcoma-Associated Herpesvirus (KSHV) gH/gL Complex Is the Predominant Neutralizing Antigenic Determinant in KSHV-Infected Individuals. Viruses, 12(3), 256.

Publication 2020

QIAamp viral RNA extraction protocol

Diagnosis Hiv 1 Hiv 2 Hiv test Plasma Quantitative real time pcr Viral rna

Corresponding Organization : University of Nebraska–Lincoln

Other organizations : Ocean Road Cancer Institute, Muhimbili University of Health and Allied Sciences

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Variable analysis

independent variables

Alere Determine HIV-1/2 Ag/Ab Combo test
HIV-1–2.0 First Response kit (Premier Medical Corporation Ltd., Mumbai, India)
QIAamp viral RNA extraction protocol (Qiagen, Hilden, Germany)
RNA Ultra-Sense One-Step quantitative real-time PCR (qPCR) system (Applied Biosystems, Foster City, CA, USA)

dependent variables

HIV-1 diagnosis
HIV-1 serology results
HIV-1 plasma viral load

control variables

HIV Rapid Test Algorithm in Zambia and Tanzania

positive controls

Not explicitly mentioned

negative controls

Not explicitly mentioned

Annotations

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