We used the primary HSC isolation and culture methods that were previously described [47 (link)]. Primary HSCs were seeded into 6-cm dishes (9 × 105 cells/dish) overnight and then transfected with a miR-29a precursor (a miR-29a mimic, GE Healthcare Dharmacon, Inc., Lafayette, CO, USA), miR-29a antisense oligonucleotide (GE Healthcare Dharmacon, Inc., Lafayette, CO, USA), or miR control (GE Healthcare Dharmacon, Inc., Lafayette, CO, USA) for 24 h using the Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, Carlsbad, CA, USA) in accordance with the manufacturer’s instructions [27 (link)].
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