Cell Viability Assay using RealTime-Glo

Following treatment, cells were transferred to 96-well plates (MDA-MB-231: 20,000 cells and MCF 10 A: 10,000 cells (as suggested by manufacturer’s protocol)) with fresh-media, as previously^{20 (link),55 (link)}. The cells were incubated for 12 h to assess the metabolic activity using RealTime-Glo MT Cell Viability Assay (Promega, USA), as per manufacturer’s protocol. Synergy LX Multi-Mode Reader (BioTek Instruments, USA) was used to record luminescence (Lum) at 1 s integration time. The sample Lum values were normalized with Ctrl to quantify viability using equation (1).

C e l l M e t a b o l i c A c t i v i t y (%) = \frac{Lum value of sample}{Lum value of control} \times 100

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Mittal L., Camarillo I.G., Varadarajan G.S., Srinivasan H., Aryal U.K, & Sundararajan R. (2020). High-throughput, Label-Free Quantitative Proteomic Studies of the Anticancer Effects of Electrical Pulses with Turmeric Silver Nanoparticles: an in vitro Model Study. Scientific Reports, 10, 7258.

Publication 2020

RealTime-Glo MT Cell Viability Assay Synergy LX Multi-Mode Reader

Assay Cell viability Cells Luminescence Promega

Corresponding Organization :

Other organizations : Purdue University West Lafayette, Anna University, Chennai, B.S. Abdur Rahman Crescent Institute of Science & Technology

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Variable analysis

independent variables

Treatment applied to cells

dependent variables

Metabolic activity of cells
Cell viability

control variables

Cell density (MDA-MB-231: 20,000 cells, MCF 10 A: 10,000 cells)
Incubation time (12 h)
Measurement technique (RealTime-Glo MT Cell Viability Assay)
Luminescence measurement (Synergy LX Multi-Mode Reader, 1 s integration time)

controls

Positive control: Not specified
Negative control: Untreated cells (Ctrl)

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