Enhancing Reprogramming Efficiency with siRNAs

1° and 2° MEFs were transfected prior to reprogramming as previously described [8 (link)]. Briefly, the cells were transfected with si-Sirt1, si-Ku70 and si-Wrn at a concentration of 100 nM (Santa Cruz Biotechnology, Texas, USA). Scrambled siRNA was used as control. Precursor of miR-135a and random sequence precursors (control) at a concentration of 50 nM (Thermo Fisher Scientific). miRCURY LNA inhibitor of miR-135a and random sequence inhibitors (control) at a concentration of 50 nM (Exiqon, Vedbaek, Denmark) were also transfected into the cells. RSV (Sigma-Aldrich) was treated at a concentration of 1 μM.