Intracerebral Pharmacological Modulation of Cognitive Processes

Scopolamine (25 μg/kg, Tocris, UK), dissolved in 0.9% saline, was injected intraperitoneally (i.p.). Muscimol (1.25 μg/2 μl, Abcam, USA) dissolved in 0.9% saline was bilaterally injected into the mPFC 1 h prior to Scopolamine. M2-AChR antagonist methoctramine (MCT, GlpBio, USA), dissolved in 0.9% saline (0.5 μg/2 μl, 1 μg/2 μl or 2 μg/2 μl), was injected intracerebroventricularly (i.c.v.) (8 (link)). M3-AChR antagonist 4-diphenylacetoxy-N-methylpiperidine methiodide (4-DAMP, 100 pmol, Cayman, USA) was dissolved in 0.9% saline (1 μg/2 μl) and injected i.c.v (8 (link)). Rapamycin (Solarbio, China), dissolved in dimethyl sulfoxide (0.2 nmol/2 μl), was delivered i.c.v. 30 min prior to MCT injection. Groups of control animals for the above experiments received equal volumes of vehicle alone (0.9% saline or dimethyl sulfoxide). The experimental procedures are illustrated in Figure 1.