Quantification of NETs in Mouse Serum

To quantify NETs in mouse serum, a capture enzyme-linked immunosorbent assay for myeloperoxidase (MPO) associated with DNA was performed as described (18 (link), 32 (link)). For the capture antibody, a mouse MPO enzyme-linked immunosorbent assay kit (Hycult Biotech; HK210-01) was used according to the manufacturer's directions. A peroxidase-labeled anti-DNA monoclonal antibody (component 2, Cell Death ELISA PLUS, catalog no. 11774424001; Roche) was used. Serum nucleosome quantification was performed using the Cell Death kit.

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Zhang H., Goswami J., Varley P., van der Windt D.J., Ren J., Loughran P., Yazdani H., Neal M.D., Simmons R.L., Zhang J., Tsung A, & Huang H. (2020). Hepatic Surgical Stress Promotes Systemic Immunothrombosis That Results in Distant Organ Injury. Frontiers in Immunology, 11, 987.

Publication 2020

HK210-01 Cell Death ELISA PLUS

Anti dna antibody Antibody Cell death Elisa Mouse Nets Nucleosome Peroxidase Serum

Corresponding Organization : Union Hospital

Other organizations : University of Pittsburgh Medical Center, Wuhan Union Hospital

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of myeloperoxidase (MPO) associated with DNA in mouse serum
Levels of serum nucleosomes

control variables

None explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

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