NF-κB Transcription Factor Assay

NF-κB DNA binding activity was performed as previously described using nuclear extracts of cells [54 (link)]. Compounds 16, 17 and 18 isolated from L. erythrocarpa were treated at a concentration of 40 μM, and cytoplasmic and nuclear fractions were obtained from an inflammatory reaction occurred cell using a nuclear extraction kit (Cayman, Ann Arbor, MI, USA). They were then analyzed at a wavelength of 450 nm using the NF-κB transcription factor assay kit (Cayman, Ann Arbor, MI, USA).

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Yoon C.S., Lee H., Liu Z., Lee H.K, & Lee D.S. (2022). Effects of Compounds Isolated from Lindera erythrocarpa on Anti-Inflammatory and Anti-Neuroinflammatory Action in BV2 Microglia and RAW264.7 Macrophage. International Journal of Molecular Sciences, 23(13), 7122.

Publication 2022

nuclear extraction kit NF-κB transcription factor assay kit

Assay Cayman Cell Cells extracts Cytoplasmic Inflammatory Nf κb Nf κb transcription factor

Corresponding Organization : Chosun University

Other organizations : Korea Research Institute of Bioscience and Biotechnology, University of Florida

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Variable analysis

independent variables

Compounds 16, 17 and 18 isolated from L. erythrocarpa
Concentration of 40 μM

dependent variables

NF-κB DNA binding activity
Cytoplasmic and nuclear fractions of cells

control variables

Nuclear extracts of cells
Inflammatory reaction occurred cell
Nuclear extraction kit (Cayman, Ann Arbor, MI, USA)
NF-κB transcription factor assay kit (Cayman, Ann Arbor, MI, USA)
Wavelength of 450 nm

controls

Positive control: Not specified
Negative control: Not specified

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