Carbon Source Evaluation for Candida glabrata

The reference strain C. glabrata ATCC 2001 was used in this study (American Type Culture Collection, Manassas, VA, USA). Standard culture media were used, including YPD (Becton, Dickinson and Company, Franklin Lakes, NJ, USA): yeast extract (1%, w/v), peptone (2%, w/v), glucose (2%, w/v), agar (1.5%, w/v), and yeast nitrogen base (YNB) without amino acids (Becton, Dickinson and Company, USA): yeast nitrogen base (0.67%, w/v), ammonium sulfate (0.5%, w/v). Synthetic complete (SC) media were prepared with YNB without amino acids, supplemented with complete supplement mixture (0.2%, w/v) (Formedium, Hunstanton, UK), glucose (2%, w/v), and agar (2%, w/v). In addition, glucose was replaced with alternative carbon sources: acetate (2%, w/v), lactate (2%, v/v), ethanol (2%, v/v), glycerol (2%, v/v), or oleic acid (0.2%, w/v) (Sigma-Aldrich, St. Louis, MO, USA) as the sole carbon source in SC media [2 (link),51 (link)].