LOH analysis for relevant MMR genes was based on the comparison of deep sequencing data for paired tumor and normal samples [37 (link)] and was performed by VarSeq (GoldenHelix®, Bozeman, MT, USA). We followed the thresholds set for putative and strict LOH in Ollikainen et al. [38 (link)]. When reporting LOH data, putative and strict LOH are called LOH for simplicity.
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