Cells were seeded at 60,000 cells per well for spontaneous migration assessment in an xCELLigence DP device (Roche Diagnostics, Mannheim, Germany) as previously reported [32] (link). The spontaneous migration was recorded for 24 hours in the presence of 5 µM fluvastatin, lovastatin, and simvastatin alone, or in combination with 100 µM trans, trans farnesol (Sigma-Aldrich Co., St. Louis, MO, USA).
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