Immunohistochemical Profiling of Neural Markers

Immunohistochemical (IH) assay was performed as described previously (Lacroix et al., 2014 (link)). Tissue sections were first blocked with 3% goat serum and then incubated with rabbit anti-BAF45D (1:100, Proteintech, Chicago, IL, United States), rabbit-anti-NESTIN (1:100, Sigma, St. Louis, MO, United States), mouse anti-NEUN (1:100, Millipore, Belecula, CA, United States) and mouse anti-beta-III-tubulin (1:200, Arigo) antibodies overnight at 4°C. Positive immunostaining was then detected using a NIKON Eclipse 80i fluorescent microscope and a NIKON Eclipse Ti-S inverted fluorescence microscope. Specific details of the animals and tissues used for IH are given in Supplementary Table S1.

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Wang Z., Huang J., Liu C., Liu L., Shen Y., Shen C, & Liu C. (2019). BAF45D Downregulation in Spinal Cord Ependymal Cells Following Spinal Cord Injury in Adult Rats and Its Potential Role in the Development of Neuronal Lesions. Frontiers in Neuroscience, 13, 1151.

Publication 2019

rabbit anti-BAF45D rabbit-anti-NESTIN mouse anti-NEUN mouse anti-beta-III-tubulin Eclipse 80i fluorescent

Animals Antibodies Assay Beta tubulin Fluorescence microscope Goat Microscope Mouse Nestin Rabbit Serum Tissues

Corresponding Organization : First Affiliated Hospital of Anhui Medical University

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Variable analysis

independent variables

Rabbit anti-BAF45D antibody (1:100)
Rabbit-anti-NESTIN antibody (1:100)
Mouse anti-NEUN antibody (1:100)
Mouse anti-beta-III-tubulin antibody (1:200)

dependent variables

Positive immunostaining

control variables

Tissue sections were first blocked with 3% goat serum

controls

Positive control: Not explicitly mentioned.
Negative control: Not explicitly mentioned.

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