After the induction of anesthesia with tribromoethanol (0.25 mg/g IP), mice were intubated and supported with a MiniVent Mouse Ventilator (Harvard Apparatus) and anesthesia was maintained with 1% isoflurane. Under sterile conditions, the heart was exposed via a left thoracotomy in the 4th intercostal space. An 8.0 prolene ligature was passed and tied around the proximal left coronary artery (HF group). In sham animals, the suture was passed but not tied. The chest was then closed using 5.0 silk. The total mice used were: C57BL/6 n = 90; TNFR1−/− n = 46; TNFR2−/− n = 39. Mice were followed for 4 weeks following operation. All TNFR1−/− and TNFR2−/− ligated mice and ∼50% WT ligated mice with premature death underwent autopsy to assess for blood in the chest cavity as an indicator of LV rupture.
Protocol detail
Murine Myocardial Infarction Model
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Anesthesia
Animals
Autopsy
Blood
Cavity of chest
Chest
Coronary artery
Heart
Isoflurane
Ligature
Mice
Prolene
Silk
Sterile
Suture
Thoracotomy
Tnfr1
Tnfr2
Tribromoethanol
Corresponding Organization :
Other organizations : University of Louisville
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Variable analysis
independent variables
- Mouse strain (C57BL/6, TNFR1-/-, TNFR2-/-)
- Survival rate
- Incidence of left ventricular (LV) rupture
- Anesthesia induction with tribromoethanol (0.25 mg/g IP)
- Anesthesia maintenance with 1% isoflurane
- Mechanical ventilation with MiniVent Mouse Ventilator (Harvard Apparatus)
- Surgical procedure (left thoracotomy in the 4th intercostal space, suture passed but not tied in sham animals)
- Positive control: C57BL/6 mice with left coronary artery ligation (HF group)
- Negative control: Sham surgery in C57BL/6 mice (suture passed but not tied)
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