This analysis of the tumors excised from mice was performed using a Zeiss LSM 710 system (Carl Zeiss), as described before (15 (link)). The primary and secondary antibodies for this experiment are listed in table S5. Apoptotic cells were detected by a standard TUNEL assay using the DeadEnd Fluorometric TUNEL System (Promega). In the fluorescence images acquired from each tissue sample, the fluorescence intensity and number of positively stained cells were quantified using the ImageJ software and presented as relative staining (%) compared to that of the PBS-treated control.
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Quantitative Fluorescence Imaging of Apoptosis
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Corresponding Organization : Ajou University
Other organizations : Samsung Medical Center, Sungkyunkwan University
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Variable analysis
independent variables
- Treatment with PBS (control)
- Fluorescence intensity
- Number of positively stained cells
- Zeiss LSM 710 system
- Primary and secondary antibodies (listed in table S5)
- TUNEL assay using DeadEnd Fluorometric TUNEL System (Promega)
- Positive control: Not specified
- Negative control: PBS-treated (control)
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