Mice were injected intraperitoneally (i.p.) twice, 14 days apart, with 100μg Ovalbumin (OVA, Sigma-Aldrich, #A5503) in 200μl containing 12.5% Imject alum adjuvant (ThermoFisher, #77161). Ten days after the last injection mice were anaesthetized with isoflurane and challenged i.n. with 30μg OVA in 30μL injection grade sterile saline. Mice were infected with SCV2 5 – 6 days after i.n. OVA challenge.
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