Histological Assessment of Colonic Inflammation

Tissues from distal colon and caecum were fixed in 4% phosphate-buffered formaldehyde solution (Fisher Scientific) for 24 hours, cut and stained with hematoxylin and eosin. Slides were blinded and scored (0-15) for parameters of inflammation and tissue damage as described in28 (link).

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Schiering C., Wincent E., Metidji A., Iseppon A., Li Y., Potocnik A.J., Omenetti S., Henderson C.J., Wolf C.R., Nebert D.W, & Stockinger B. (2017). Feedback Control of AHR Signaling Regulates Intestinal Immunity. Nature, 542(7640), 242-245.

Publication 2017

phosphate-buffered formaldehyde solution

Caecum Colon Eosin Formaldehyde solution Inflammation Phosphate Tissue

Corresponding Organization :

Other organizations : The Francis Crick Institute, University of Edinburgh, University of Dundee, University of Cincinnati

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Scores (0-15) for parameters of inflammation and tissue damage

control variables

Tissue samples from distal colon and caecum
Fixation in 4% phosphate-buffered formaldehyde solution for 24 hours
Staining with hematoxylin and eosin

controls

Positive control: Not mentioned
Negative control: Not mentioned

Annotations

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