Isolation and Culture of Human Skin Fibroblasts

All recruited individuals signed an informed consent form approved by our Institutional Ethics Committees prior to being enrolled in the study on a voluntary basis (Ethical research board of the CHU de Québec. Protocol number: 2012–1316. For more information, please contact (gurecherche@chuq.qc.ca). All experiments were performed in compliance with the national Tri-Council Policy Guidelines: Ethical Conduct for Research Involving Humans and approved by the ethics committee of the CHU de Quebec – Université Laval. Fibroblasts were isolated from skin biopsies of 6 informed consent healthy individuals (Supplementary Table 5) as previously described^{33 (link)}. Fibroblasts were grown in Dulbecco-Vogt modification of Eagle’s medium (DMEM; Invitrogen, Burlington, ON, Canada) supplemented with 10% foetal bovine serum (FBS; VWR, Radnor, PA, USA), 100 IU/mL penicillin G (Sigma-Aldrich, Oakville, QC, Canada), and 25 μg/mL gentamicin (Schering, Pointe-Claire, QC, Canada). Cell cultures were kept in an incubator at 37 °C, 8% CO₂ and 95% relative humidity, and media was changed every 2 days.