Oxygen-Glucose Deprivation Induces Neuronal Injury

The HT22 cells were obtained from Procell Life Science & Technology (China; CL-0595) and cultured in DMEM with 10% FBS at 37°C. To induce OGD, the neurons were cultured in glucose-free DMEM solution and maintained in a hypoxic chamber (Thermo Fisher Scientific) at 94% N₂, 1% O₂, 5% CO₂ and 37°C for 2, 4, 8, or 12 h, as previously described (Chen et al., 2011 (link); Xu et al., 2018 (link)). The HT22 cells in the normal group were subjected to OGD for 0 h. After OGD, the neurons were cultured in a normal DMEM solution with 10% FBS for 24 h or 48 h. After 24 h or 48 h of re-oxygenation, neurons were subjected to later assay.