Samples from the Oroboros O2k chambers were collected at the end of each experiment and stored in -80°C until measurement. Citrate synthase activity was assessed as previously described (27 (link)) using 300 μg of platelets protein. Since the irreversible chemical reaction CoA-SH + DTNB → TNB + CoA-S-S-TNB is catalyzed by citrate synthase, the amount of thionitrobenzoic acid (TNB) was quantified by measuring absorbance at 412 nm using Cytation5 Cell Imaging Reader (Agilent BioTek, CA, USA).
Yasseen B.A., Elkhodiry A.A., El-Messiery R.M., El-sayed H., Elbenhawi M.W., Kamel A.G., Gad S.A., Zidan M., Hamza M.S., Al-ansary M., Abdel-Rahman E.A, & Ali S.S. (2022). Platelets’ morphology, metabolic profile, exocytosis, and heterotypic aggregation with leukocytes in relation to severity and mortality of COVID-19-patients. Frontiers in Immunology, 13, 1022401.
No positive or negative controls were explicitly mentioned in the protocol.
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