Biparental LsAPRR2 Promoter GUS Assay
Corresponding Organization : Guangxi University
Variable analysis
- Fusion reporter vectors (containing the LsAPRR2 promoter cloned into pBI121 with GUS reporter gene)
- Empty vector (pBI121)
- GUS reporter gene expression
- Tobacco leaves
- Incubation time (7 days after injection)
- X-Gluc buffer composition (12 mM potassium ferricyanide, 0.3% (v/v) Triton X-100, 12 mM potassium ferrocyanide, 1 mg/ml 5-bromo-4-chloro-3-indolyl-β-D-glucuronide)
- Staining conditions (vacuum infiltration, overnight incubation at 37°C)
- Decolorization process (several washes in 75% (v/v) ethanol)
Annotations
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