In order to investigate the activity of PrWRI1 visually, the coding sequence of PrWRI1 was cloned into the SacII and BamHI restriction sites of pK34 entry vector, and then the recombinant pK34 vector with double CaMV 35S promoters and a terminator sequence was digested with AscI for entry into plant expression vector, pB110. The vector was then transiently expressed in Nicotiana benthamiana leaves by Agrobacterium-mediated transformation.
The ORF of PrWRI1 were inserted into the vector pCAMBIA1300 under the control of Arabidopsis seed-specific promoter 2S2 by the digestion of KpnI and BamHI. The generated constructs were transformed into Agrobacterium tumefaciens GV3101 using the freeze–thaw method, and then they were used for the transformation of wild-type Arabidopsis by the floral dip method.
The ORF of PrWRI1 were inserted into the vector pCAMBIA1300 under the control of Arabidopsis seed-specific promoter 2S2 by the digestion of KpnI and BamHI. The generated constructs were transformed into Agrobacterium tumefaciens GV3101 using the freeze–thaw method, and then they were used for the transformation of wild-type Arabidopsis by the floral dip method.
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