Male A/J (A; n = 19), BALB/cByJ (BALB; n = 18), C3H/HeJ (C3H; n = 18), C57BL/6J (B6; n = 18), CBA/J (CBA; n = 18), DBA/1J (D1; n = 20), and DBA/2J (D2; n = 18) mice were obtained from Jackson Laboratory (Bar Harbor, ME). 129/SvEv (129) mice were obtained from Taconic Farms (Germantown, NY). These inbred strains were chosen because previous work from our lab found phenotype differences in these strains for the effects of nicotine on learning and memory (Portugal, Wilkinson, Kenney, Sullivan, & Gould, 2012 (link)). Mice were trained at 8–10 weeks of age. Mice were housed in groups of three per cage and maintained on a 12-hr light–dark cycle (lights on 7:00 a.m.) with unrestricted access to food and water. Experiments took place during the light phase. All behavioral and surgical procedures were approved by the Temple University Institutional Animal Care and Use Committee.
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Strain-Specific Effects of Nicotine on Learning
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Variable analysis
independent variables
- Mouse strain
- Effects of nicotine on learning and memory
- Age of mice (8-10 weeks)
- Housing conditions (3 mice per cage)
- Light-dark cycle (12-hr light-dark cycle, lights on at 7:00 a.m.)
- Access to food and water (unrestricted)
- Timing of experiments (during light phase)
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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