Four ml of 5 × 105 cells/well were seeded in a 6-well plate the night before the treatment. Cells were treated with camptothecin at a final concentration of 6 μM for 4 hr in the 37°C incubator before the cells were subjected to EB/AO staining. Then, 1 ml of cell suspension was used for conventional EB/AO staining, and 100 μl of cell suspension was transferred to a 96-well plate for modified EB/AO staining.
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