Optimized 2D Gel Protocol for Zebrafish
Corresponding Organization :
Other organizations : Max Planck Institute of Molecular Cell Biology and Genetics
Protocol cited in 39 other protocols
Variable analysis
- Optimized conditions and settings for zebrafish samples
- Protein separation and visualization on 2D gel electrophoresis
- Removal of supernatant after the last washing step of the deyolking procedure to keep the salt concentration as low as possible
- Total protein precipitation to reduce salts and other interfering substances (may be applied for preparative gels)
- No positive or negative controls were explicitly mentioned.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!