Quantitative Determination of CAA and CCA

As a reference standard for the quantitative determination of CAA and CCA, a TCA-soluble fraction of S. mansoni adult worm antigen, AWA-TCA (Deelder et al. 1980 (link)), was used in a similar manner as described by Polman et al. (2000) (link). Soluble egg antigen (SEA) was prepared following similar protocols as for AWA, using S. mansoni eggs recovered from the livers of infected hamsters. Soluble crude cercarial antigen preparation (SCAP) was prepared from freshly shed cercariae, collected after precipitation under gravity in ice-cooled water, and subsequently treated similarly to SEA or AWA.

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CORSTJENS P.L., DE DOOD C.J., KORNELIS D., FAT E.M., WILSON R.A., KARIUKI T.M., NYAKUNDI R.K., LOVERDE P.T., ABRAMS W.R., TANKE H.J., VAN LIESHOUT L., DEELDER A.M, & VAN DAM G.J. (2014). Tools for diagnosis, monitoring and screening of Schistosoma infections utilizing lateral-flow based assays and upconverting phosphor labels. Parasitology, 141(14), 1841-1855.

Publication 2014

Adult Antigen Cercariae Eggs Gravity Hamsters Livers Water ice Worm antigen

Corresponding Organization :

Other organizations : Leiden University Medical Center, University of York, National Museums of Kenya, Institute of Primate Research, The University of Texas Health Science Center at San Antonio, New York University

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Variable analysis

independent variables

TCA-soluble fraction of S. mansoni adult worm antigen (AWA-TCA)
Soluble egg antigen (SEA)
Soluble crude cercarial antigen preparation (SCAP)

dependent variables

Quantitative determination of CAA and CCA

control variables

The preparation of AWA-TCA, SEA, and SCAP was carried out in a similar manner as described by the referenced studies

controls

AWA-TCA was used as a reference standard for the quantitative determination of CAA and CCA

Annotations

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