Lip(PFH) was prepared by ultrasonication according to our previously reported study [15 (link)]. Briefly, liposome colloidal suspensions were prepared by dissolving 3.79 mg DSPE-PEG2000 (4.28 mg cholesterol and 24.65 mg lecithin in dichloromethane. The organic solvent was removed through rotary evaporation to form a thin lipids film on the glass vial. Thereafter, the lipid film was hydrated with 1.4 ml pure water by ultrasonication (XO-650D, China) for 10 min in ice bath. After that, 0.6 ml PFH were gradually appended under ultrasonication at 325 w/min for 3 min in ice bath to form 2 ml lip(PFH) (30 v/v % PFH). For in vivo tracking, IR780, a near infrared (NIR) dye, was added in dichloromethane to form lip(PFH + IR780) [15 (link)].
The morphology of lip(PFH) was characterized by transmission electron microscopy (TEM, H-7650, Hitachi, Japan). Particle size distribution of liposomes was detected by Nanoparticle Size Analyzer (ZEN3600, Malvern). B ultrasound was used to further confirm that PFH was encapsulated into liposome.
The morphology of lip(PFH) was characterized by transmission electron microscopy (TEM, H-7650, Hitachi, Japan). Particle size distribution of liposomes was detected by Nanoparticle Size Analyzer (ZEN3600, Malvern). B ultrasound was used to further confirm that PFH was encapsulated into liposome.
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