Siderophore production was investigated using Chrome azurol S (CAS) agar (1.21 g Chrome azurol S (CAS), 100 mL iron (III) solution (1 mmol FeCl 3 6H 2 O, 10 mmol HCl), 1.82 g hexadecyltrimetyl ammonium bromide (HDTMA), 0.3 % (v/v) methylene blue, 15 g agar per liter of water). The medium was prepared according to the method described by [36] . Yeasts were grown on YPD agar at 25 ± 2 °C for 24 h, and then a single colony was picked and point inoculated onto CAS agar. The cultures were incubated at 25 ± 2 °C for 5 days. The isolate showed colonies surrounded by light orange zones, indicating the presence of iron carriers.
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Siderophore Production Assay using CAS
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Other organizations : Kasetsart University
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Variable analysis
independent variables
- Siderophore production
- Formation of light orange zones around the colonies, indicating the presence of iron carriers
- Growth of yeasts on YPD agar at 25 ± 2 °C for 24 h
- Incubation of the cultures on CAS agar at 25 ± 2 °C for 5 days
- No positive or negative controls were explicitly mentioned in the information provided.
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