Physicochemical Characterization of Diverse Silica Particles

Crystalline silica particles (DQ12; DMT GmbH and Co. KG, Essen, Germany), Vitreous silica (VS; a gift from Dr. Ghiazza, University of Torino, Italy), Aerosil 200 (Sigma-Aldrich, Bornem, Belgium) and multi-wall carbon nanotubes (MWCNT; MWNT-7, Mitsui, Tokyo, Japan) powders were sterilized by heating at 200°c for 2h immediately before suspension. Sterile Stöber particle suspension were synthesized based on Stöber process and its characterization was described elsewhere [81 (link)]. Characteristics of the particles are listed in Table 1.Table 1

Physicochemical characterization of the particles

Name	Type	Crystallinity	Size TEM (nm)	Surface BET (m²/g)
DQ12	Ground natural mineral	Crystalline	960^a	7,4 -10,1^b
VS	Ground fused silica	Amorphous	1600^c	3.1^c
Stöber	Stöber silica	Amorphous	12^d	400^d
Aerosil 200	Fumed silica	Amorphous	12^e	175-225^e
MWCNT	Multi-wall carbon nanotubes	N/a	Length :5,700 ± 3,700^f	22^f
MWCNT	Multi-wall carbon nanotubes	N/a	Width : 74 ± 28^f	22^f

(a) [82 (link)].

(b) [83 (link)].

(d) [84 (link)].

(e) Data provided by the manufacturer (Sigma-Aldrich).

(f) [85 (link)].

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Rabolli V., Badissi A.A., Devosse R., Uwambayinema F., Yakoub Y., Palmai-Pallag M., Lebrun A., De Gussem V., Couillin I., Ryffel B., Marbaix E., Lison D, & Huaux F. (2014). The alarmin IL-1α is a master cytokine in acute lung inflammation induced by silica micro- and nanoparticles. Particle and Fibre Toxicology, 11, 69.

Publication 2014

Aerosil 200

Aerosil Carbon Carbon nanotubes Powders Silica Sterile

Corresponding Organization : UCLouvain

Other organizations : Immunologie et Neurogénétique Expérimentales et Moléculaires, Université d'Orléans, Centre National de la Recherche Scientifique, de Duve Institute

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Variable analysis

independent variables

Crystalline silica particles (DQ12)
Vitreous silica (VS)
Aerosil 200
Multi-wall carbon nanotubes (MWCNT)

dependent variables

Not explicitly mentioned

control variables

Heating the powders at 200°C for 2 hours before suspension
Synthesizing sterile Stöber particle suspensions based on the Stöber process

positive controls

None mentioned

negative controls

None mentioned

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