Immunohistochemical Staining of Tumor Samples

Tumors were fixed in formalin, embedded in paraffin, and sectioned for staining. Staining was done as previously described (26 (link)) with anti-p-Stat3 (1:100, Abcam), anti-PCNA (1:100, Abcam), and anti-Mcl-1 (1:100, Abcam) per the manufacturer’s instructions.

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Quinn B.A., Dash R., Sarkar S., Azab B., Bhoopathi P., Das S.K., Emdad L., Wei J., Pellecchia M., Sarkar D, & Fisher P.B. (2015). Pancreatic cancer combination therapy using a BH3 mimetic and a synthetic tetracycline. Cancer research, 75(11), 2305-2315.

Publication 2015

anti-p-Stat3 anti-PCNA anti-Mcl-1

Embedded paraffin Formalin Pcna Stat3 Tumors

Corresponding Organization : Virginia Commonwealth University

Other organizations : Institute of Life Sciences, University of Jordan, Sanford Burnham Prebys Medical Discovery Institute

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Variable analysis

independent variables

Staining method: anti-p-Stat3 (1:100, Abcam), anti-PCNA (1:100, Abcam), and anti-Mcl-1 (1:100, Abcam)

dependent variables

Expression levels of p-Stat3, PCNA, and Mcl-1 proteins

control variables

Tumor samples were fixed in formalin and embedded in paraffin
Staining was done per the manufacturer's instructions

controls

No positive or negative controls were explicitly mentioned

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