Drosophila Genetic Manipulation Protocol

The following stocks were used: pnr-GAL4 (Bloomington:3039), aPKC^ts (Guilgur et al., 2012 (link)), cdc42³ (Fehon et al., 1997 (link)), tub-GAL80^ts (Bloomington:7108), UAS-Dia^CA (Bloomington:27616), UAS-Cdc42^V12 (Bloomington:6287), neu-GMA (Edwards et al., 1997; Kunda et al., 2012 ), neu-GAL4 (Bellaïche et al., 2001 (link)), Lifeact::GFP (Hatan et al., 2011 (link)), sqh^Ax3; Sqh::GFP (Monier et al., 2010 (link)), Cdc42^V5 (Fletcher et al., 2012 (link)), UAS-Tub::RFP (McGill et al., 2009 (link)), and UAS-Pbl::GFP (Somers and Saint, 2003 (link)). Vienna Drosophila Resource Center (VDRC) RNAi lines were used to silence the expression of the genes aPKC, arp3, diaphanous, pebble, and par-6, and a NIG-Fly library line was used for cdc42. For Flybase ID, see Supplemental Experimental Procedures.

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Rosa A., Vlassaks E., Pichaud F, & Baum B. (2015). Ect2/Pbl Acts via Rho and Polarity Proteins to Direct the Assembly of an Isotropic Actomyosin Cortex upon Mitotic Entry. Developmental Cell, 32(5), 604-616.

Publication 2015

pnr-GAL4 tub-GAL80ts UAS-DiaCA UAS-Cdc42V12

Cdc42 Center lines Diaca Drosophila Expression genes Library Rnai

Corresponding Organization : MRC Laboratory for Molecular Cell Biology

Other organizations : Universidade do Porto

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Variable analysis

independent variables

Pnr-GAL4
APKC^ts
Cdc42^3
Tub-GAL80^ts
UAS-Dia^CA
UAS-Cdc42^V12
RNAi lines for aPKC, arp3, diaphanous, pebble, and par-6
NIG-Fly library line for cdc42

dependent variables

Neu-GMA
Lifeact::GFP
Sqh^Ax3; Sqh::GFP
Cdc42^V5
UAS-Tub::RFP
UAS-Pbl::GFP

control variables

Neu-GAL4

controls

Positive controls not explicitly mentioned.
Negative controls not explicitly mentioned.

Annotations

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