Dietary Modulation of Cholesterol Metabolism

0.5% (w/w) CA in Teklad 7001 or Teklad 2019 base diet was from Envigo. 2% (w/w) cholestyramine (Sigma) diet on AIN93G was made by Research Diets. 0.2% (w/v) sodium deoxycholic acid (Sigma) was dissolved in sterile water. Tamoxifen (Sigma) treatment was performed by intraperitoneal (IP) injection or given in chow (Envigo TD.130858) (64 (link)), as indicated in legends. Tamoxifen was dissolved in ethanol/cremaphore at 20 mg/ml, diluted 1:1 in sterile PBS and mice received 1 mg in 100 ul. Tamoxifen chow was provided for 2–4 wks. Labelling was assessed 24 hrs after last injection/removal of diet. Diphtheria toxin (Sigma) was injected retro-orbitally, 500 ng/100 ul sterile PBS/mouse. Two injections were given 48 hrs apart, and mice were analyzed for deletion after 24 hrs. GW4064 (Selleck Chem) was dissolved in DMSO, resuspended in sterile methycellulose and given IP at 100 mg/kg body weight.

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O’Leary C.E., Sbierski-Kind J., Kotas M.E., Wagner J.C., Liang H.E., Schroeder A.W., de Tenorio J.C., von Moltke J., Ricardo-Gonzalez R.R., Eckalbar W.L., Molofsky A., Schneider C, & Locksley R.M. (2022). Bile acid-sensitive tuft cells regulate biliary neutrophil influx. Science immunology, 7(69), eabj1080.

Publication 2022

Teklad 2019 cholestyramine sodium deoxycholic acid Tamoxifen Diphtheria toxin GW4064

Body weight Cholestyramine Deletion Deoxycholic acid Diet Diphtheria toxin Dmso Ethanol Gw4064 Intraperitoneal injection Mice Sodium Sterile Tamoxifen

Corresponding Organization : Howard Hughes Medical Institute

Other organizations : University of Zurich, University of Washington

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Variable analysis

independent variables

0.5% (w/w) CA in Teklad 7001 or Teklad 2019 base diet
2% (w/w) cholestyramine (Sigma) diet on AIN93G
0.2% (w/v) sodium deoxycholic acid (Sigma) dissolved in sterile water
Tamoxifen (Sigma) treatment by intraperitoneal (IP) injection or given in chow (Envigo TD.130858)
Diphtheria toxin (Sigma) injected retro-orbitally
GW4064 (Selleck Chem) dissolved in DMSO, resuspended in sterile methycellulose and given IP at 100 mg/kg body weight

dependent variables

Labelling assessed 24 hrs after last injection/removal of diet

control variables

Teklad 7001 or Teklad 2019 base diet
AIN93G diet
Sterile water
Ethanol/cremaphore
Sterile PBS
Sterile methycellulose

Annotations

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