Tea leaves, rhubarb rhizome, white mustard, and black seeds were purchased as dried material, and aerial parts of R. graveolens, E. vesicaria, and A. officinalis, grapefruit peels, and grapes were dried in a vacuum-heated oven at 40 °C. All dried materials were powdered, and 50 g of each dried powder was repeatedly extracted with methanol (Adwic Co., Cairo, Egypt) until exhaustion (200 mL × 4) to obtain the methanolic extract. The fruits were peeled, thinly sliced, and dried in a vacuum oven for avocado. The dried fruits were powdered and then extracted with n-hexane (200 mL × 4, Adwic Co.) to obtain the fixed oil. Solvents were removed using a rotary evaporator, and the obtained residues were stored in amber-colored glass at 4 °C until use.
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