Human neuroblastoma cells (SH-SY5Y, ATCC CRL-2266) were cultured in Dulbecco's Modified Essential (DMEM) and F12 medium (GIBCO, supplemented with 10% fetal bovine serum (FSB, HyClone, Logan, Utah) and 100 U/mL penicillin‒streptomycin (P/S; GIBCO).
Human neural stem cells (NSCs) derived from iPS cells were prepared as previously described [2 (link)]. Human iPS cells were cultured in PSC neural induction medium (GIBCO, USA) containing neurobasal medium and PSC supplement for 7 days. Then, initial neural stem cells (NSCs) were split and expanded on neural induction medium (advanced DMEM/F12 and neurobasal medium (1:1) with neural induction supplement; Gibco).
African green monkey kidney (VERO subtype E6) cells were maintained in high glucose DMEM supplemented with 10% FBS and 100 U/mL P/S. All cell types were maintained at 37 °C in 5% CO2.
Human neural stem cells (NSCs) derived from iPS cells were prepared as previously described [2 (link)]. Human iPS cells were cultured in PSC neural induction medium (GIBCO, USA) containing neurobasal medium and PSC supplement for 7 days. Then, initial neural stem cells (NSCs) were split and expanded on neural induction medium (advanced DMEM/F12 and neurobasal medium (1:1) with neural induction supplement; Gibco).
African green monkey kidney (VERO subtype E6) cells were maintained in high glucose DMEM supplemented with 10% FBS and 100 U/mL P/S. All cell types were maintained at 37 °C in 5% CO2.
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