Bacterial Culture Protocols for Bordetella spp.

Wild-type Bordetella bronchiseptica strain RB50 (B. bronchiseptica) and a derivative btrS mutant (RB50ΔbtrS) (3 (link)), here called Bbvac (52 ), B. pertussis, and B. parapertussis were grown on plates of Difco Bordet-Gengou agar (BD; catalog no. 248200) supplemented with 20% defibrinated sheep blood from HemoStat and 20 μg/mL of streptomycin as previously described (1 (link)). For liquid cultures, B. pertussis and B. parapertussis were grown in Stainer-Scholte medium (1 (link)). B. bronchiseptica and Bbvac were grown in LB media (1 (link)). Details of the clinical strains included in this study are found in Table S2 in the supplemental material.

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Gestal M.C., Howard L.K., Dewan K.K, & Harvill E.T. (2022). Bbvac: A Live Vaccine Candidate That Provides Long-Lasting Anamnestic and Th17-Mediated Immunity against the Three Classical Bordetella spp.. mSphere, 7(1), e00892-21.

Publication 2022

Bordet-Gengou agar

Agar B pertussis Blood Bordetella bronchiseptica Hemostat Sheep Strains Streptomycin

Corresponding Organization : University of Georgia

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Variable analysis

independent variables

Wild-type Bordetella bronchiseptica strain RB50 (B. bronchiseptica)
Derivative btrS mutant (RB50ΔbtrS), here called Bbvac
B. pertussis
B. parapertussis

dependent variables

Not explicitly mentioned

control variables

Bordet-Gengou agar supplemented with 20% defibrinated sheep blood and 20 μg/mL of streptomycin for plate cultures
Stainer-Scholte medium for B. pertussis and B. parapertussis liquid cultures
LB media for B. bronchiseptica and Bbvac liquid cultures

controls

Positive control: Wild-type B. bronchiseptica strain RB50 (B. bronchiseptica)
Negative control: Not explicitly mentioned

Annotations

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