Serum interleukin-9 (IL-9), interleukin-1β (IL-1β), macrophage inflammatory protein-1α (MIP-1α), angiogenin, granulocyte colony-stimulating factor (G-CSF), interleukin-1α (IL-1α), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein-1β (MIP-1β), immunoglobulin E (IgE), interleukin-6 (IL-6), fractalkine, interleukin-8 (IL-8), tumor necrosis factor-α (TNF-α), monokine induced by interferon-gamma (MIG), rantes, and granzyme B were analyzed by multiparameter flow cytometry using the corresponding antibodies from BD Biosciences (East Rutherford, NJ, United States) and the BD FACSymphonyA5 (BD Biosciences).
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