Cytokine Profiling in Murine Lungs

In experiment 2, the water intake, food intake, and body weight of mice were recorded daily. The right lung lobes of mice were collected to prepare 10% lung homogenates by using an electric homogenizer on ice, and the homogenates were stored at –80 °C for cytokine assays [33 (link)]. The levels of tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), IL-4, IL-5, IL-10, IL-12p70, interferon-γ (IFN-γ), and granulocyte–macrophage colony-stimulating factor (GM-CSF) in lung homogenates were detected by Luminex Liquid Chip Technology in accordance with the manufacturer’s instructions (Bio-Plex Pro™ Mouse Cytokine, Bio-Rad).