Isonicotinic acid hydrazide (isoniazid, INH) , ethionamide (ETH) , and (S,S) -N,Nʼ-bis (1-hydroxy-2-butyl) -ethylenediamine dihydrochloride (ethambutol dihydrochloride, EB) were obtained from Tokyo Chemical Industry Co., Ltd.
(Tokyo, Japan) . The antimicrobial activities of these antitubercular agents against Mycobacterium spp. and Legionella spp. were tested. INH and EB were dissolved in water and