Pharmacological Modulation of DMD Macrophages

JWH-133 and AM630 powder were purchased from Tocris (Avonmouth, UK) and were dissolved in PBS containing DMSO. DMSO final concentration on cultures was 0.01%. DMD-associated macrophages were treated with JWH-133 [100 nM] and AM630 [10 µM] for 24 h. Non-treated cultured macrophages were maintained in incubation media during the relative treatment time with or without vehicle (DMSO 0.01%).

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Argenziano M., Pota V., Di Paola A., Tortora C., Marrapodi M.M., Giliberti G., Roberti D., Pace M.C, & Rossi F. (2023). CB2 Receptor as Emerging Anti-Inflammatory Target in Duchenne Muscular Dystrophy. International Journal of Molecular Sciences, 24(4), 3345.

Publication 2023

JWH-133 AM630

Am630 Dmso Jwh 133 Macrophages Powder

Corresponding Organization : University of Campania "Luigi Vanvitelli"

Other organizations : Centro Clinico Nemo

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Variable analysis

independent variables

JWH-133 [100 nM]
AM630 [10 μM]

dependent variables

Changes in DMD-associated macrophages

control variables

DMSO final concentration on cultures was 0.01%
Non-treated cultured macrophages were maintained in incubation media during the relative treatment time with or without vehicle (DMSO 0.01%)

controls

Positive control: Non-treated cultured macrophages were maintained in incubation media during the relative treatment time.
Negative control: Non-treated cultured macrophages were maintained in incubation media during the relative treatment time with vehicle (DMSO 0.01%).

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