Total soluble protein was assayed using Pierce Bicinchoninic Acid (BCA) protein assay (ThermoFisher, USA) to establish the optimal approach for recovering the total amount of soluble protein from the flesh of snakehead fish. Its absorbance was measured at 540 nm in an ELISA plate reader. Bovine serum albumin (BSA) was used as the standard protein. The analysis was performed following the protocol provided by ThermoFisher Scientific. The ratio 1:8 of sample to working reagent was prepared, loaded into a 96-well plate, and incubated at 37 °C for 30 min. The total soluble protein concentration was quantified according to the standard curve obtained from the protein standard.
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Optimizing Soluble Protein Extraction from Snakehead Fish
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Corresponding Organization : Chiang Mai University
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Variable analysis
independent variables
- Approach for recovering the total amount of soluble protein from the flesh of snakehead fish
- Total soluble protein concentration
- Ratio of sample to working reagent (1:8)
- Incubation temperature (37 °C)
- Incubation time (30 min)
- Bovine serum albumin (BSA) as the standard protein
- Positive control: Bovine serum albumin (BSA) used as the standard protein
- Negative control: Not explicitly mentioned
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