Cerebrospinal Fluid Biomarker Measurement

Cerebrospinal fluid collection procedures can be obtained from the ADNI database and prior publications (ADNI_Methods_UPENN_Biomarker_20120710.pdf) (Shaw et al., 2011 (link)). The xMAP Luminex platform (flow cytometric method) and Innogenetics/Fujirebio AlzBio3 immunoassay kits (monoclonal assay) were used following procedures in place at the UPenn/ADNI Biomarker Laboratory, according to the kit manufacturer’s instructions and as described in previous publications (Shaw et al., 2011 (link)). These assays produced measurements for Aβ_1-42, total tau, and p-tau₁₈₁. In this study, we used total CSF Aβ_1-42 as a variable of interest.

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Scott J.A., Braskie M.N., Tosun D., Thompson P.M., Weiner M., DeCarli C, & Carmichael O.T. (2015). Cerebral Amyloid and Hypertension are Independently Associated with White Matter Lesions in Elderly. Frontiers in Aging Neuroscience, 7, 221.

Publication 2015

xMAP Luminex platform AlzBio3 immunoassay kits

Assays Biomarker Cerebrospinal fluid Flow cytometric Immunoassay

Corresponding Organization : University of California, Davis

Other organizations : University of Southern California, San Francisco VA Medical Center, University of California, San Francisco, Louisiana State University, Pennington Biomedical Research Center

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Total CSF Aβ1-42

control variables

Procedures for cerebrospinal fluid collection and biomarker measurements using the xMAP Luminex platform and Innogenetics/Fujirebio AlzBio3 immunoassay kits were controlled and kept constant according to the protocols in place at the UPenn/ADNI Biomarker Laboratory, as described in previous publications.

controls

No specific positive or negative controls were mentioned in the provided information.

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