Immunohistochemical Analysis of Cellular Markers

Immunohistochemical staining, evaluation of the results, and calculation of the mean density of staining was performed as previously described [14 (link), 23 (link)]. The primary antibodies were incubated as follows: mouse monoclonal anti-Galectin-1 (sc-166618; 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-E-cadherin (sc-52327; 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti- Vimentin (sc-6260; 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and anti-MMP9 (sc-21733; 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), anti-NF-кB p65 (sc-8008; 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), and anti-Twist (sc-81417; 1:200; Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).

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Tang D., Zhang J., Yuan Z., Zhang H., Chong Y., Huang Y., Wang J., Xiong Q., Wang S., Wu Q., Tian Y., Lu Y., Ge X., Shen W, & Wang D. (2017). PSC-derived Galectin-1 inducing epithelial-mesenchymal transition of pancreatic ductal adenocarcinoma cells by activating the NF-κB pathway. Oncotarget, 8(49), 86488-86502.

Publication 2017

anti-Galectin-1 anti-E-cadherin anti- Vimentin anti-MMP9 (sc-21733 anti-NF-кB p65

Antibodies Cadherin Galectin 1 Mmp9 Mouse Vimentin

Corresponding Organization :

Other organizations : Yangzhou University, Anhui Provincial Hospital, Nanjing Medical University, Jiangsu Province Hospital

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Variable analysis

independent variables

Immunohistochemical staining protocol
Primary antibodies (mouse monoclonal anti-Galectin-1, anti-E-cadherin, anti-Vimentin, anti-MMP9, anti-NF-кB p65, and anti-Twist)

dependent variables

Evaluation of the results
Calculation of the mean density of staining

control variables

Incubation of primary antibodies (1:200 dilution)

controls

No specific positive or negative controls were mentioned.

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