Heterologous Protein Expression in Yeast

The plasmid pPIC9K and the yeast cell strain GS115 were obtained from the Key Laboratory of Molecular Medicine of Fudan University [15 (link)]. The E. coli strain DH5α was obtained from TIANGEN Biotech Co., Ltd. (Beijing).
Yeast nitrogen base (with or without amino acids) was purchased from Sigma. Q-Sepharose-FF and Sephadex G-50 were purchased from GE Healthcare.

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Wei Y., Zhan Y., Chen P., Liu Z., Zhang H., Liu D., Zhang J., Yu M., Mo W., Zhang J, & Zhang X. (2017). Heterologous expression, purification and function of the extracellular domain of human RANK. BMC Biotechnology, 17, 87.

Publication 2017

Yeast nitrogen base Q-Sepharose-FF Sephadex G-50

Amino acids Cell strain E coli Nitrogen Plasmid Sephadex g 50 Sepharose Strain Yeast

Corresponding Organization : Shanghai Jiao Tong University

Other organizations : First Affiliated Hospital of Bengbu Medical College, Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Fudan University

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Variable analysis

independent variables

The plasmid pPIC9K
The yeast cell strain GS115
The E. coli strain DH5α

dependent variables

Not explicitly mentioned

control variables

Yeast nitrogen base (with or without amino acids)
Q-Sepharose-FF
Sephadex G-50

controls

No positive or negative controls were explicitly mentioned in the provided information.

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