Characterize Rat Neural Progenitor Cells

According to the prior studies, three major specific cell surface markers of rat NPCs (N-CDH, KRT19, and SOX9) were identified by the fluorescence activated cell sorter (FACS) using flow cytometry 20 (link)-22 (link). Briefly, the isolated cells were trypsinized, centrifuged and resuspended in PBS. Then the cells were fixed and permeabilized by fixation and permeabilization kit, and incubated successively with primary antibodies (anti-N-cadherin (N-CDH, Proteintech, China), anti-keratin19 (KRT19, Proteintech, China), and anti-SOX9 (Abcam, USA)), and FITC-labeled secondary antibody (Abcam, USA). Finally, the expression of fluorescent intensity was quantitatively determined by the flow cytometry.