Myocardial Infarction in C57Bl/6 Mice

Myocardial infarction (MI) of C57Bl/6 mice at age of 8 weeks was induced as previously described^{17 (link)}. Briefly, general anesthesia was performed with 1-2% isoflurane and maintained on a ventilator. The heart was exposed to a left open thoracotomy, and the left anterior descending branch (LAD) was ligated with a 6-0 polyester thread 1 mm from the normal position of the left pinna tip. The surgical wound was sutured, and animals were allowed to recover. For the fumarate supplementation, the sodium fumarate dibasic (Sigma-Aldrich, F1506) was admitted to Sham and MI group at the dose of 2 g/L through drinking water 1 day prior to the surgery and lasted 7 days after the surgery.

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Wang X., Zhang X., Cao K., Zeng M., Fu X., Zheng A., Zhang F., Gao F., Zou X., Li H., Li M., Lv W., Xu J., Long J., Zang W., Chen J., Gao F., Ding J., Liu J, & Feng Z. (2022). Cardiac disruption of SDHAF4-mediated mitochondrial complex II assembly promotes dilated cardiomyopathy. Nature Communications, 13, 3947.

Publication 2022

sodium fumarate dibasic

Animals C57bl mice Fumarate General anesthesia Heart Isoflurane Myocardial infarction Pinna Polyester Sodium fumarate Surgical wound Thoracotomy

Corresponding Organization : Xi'an Jiaotong University

Other organizations : Air Force Medical University, Second Affiliated Hospital of Zhejiang University, Second Affiliated Hospital of Xi'an Jiaotong University

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Variable analysis

independent variables

Myocardial infarction (MI) induction in C57Bl/6 mice at 8 weeks of age
Fumarate supplementation through drinking water (2 g/L) starting 1 day prior to surgery and lasting for 7 days after surgery

dependent variables

Outcome measures (not explicitly mentioned)

control variables

General anesthesia with 1-2% isoflurane and ventilation
Left open thoracotomy and ligation of the left anterior descending branch (LAD) 1 mm from the left pinna tip
Surgical wound suturing and animal recovery

controls

Sham group (underwent the surgical procedure without LAD ligation)

Annotations

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