Molecular Typing of Staphylococcus aureus

Spa typing was performed using PCR followed by Sanger sequencing, as previously described [24 (link)]. Assignment of spa type Based Upon Repeat Pattern (BURP) analysis for determination of spa clonal complex (spa-CC) was performed using Ridom StaphType. The Ridom SpaServer was used to predict the multi-locus sequence types (STs) as described previously [25 (link)]. Phylogenetic trees were constructed using RAxML Tree, Geneious Version 2022.2, (https://www.geneious.com).

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Ibrahim R.A., Mekuria Z., Wang S.H., Mediavilla J.R., Kreiswirth B., Seyoum E.T., Mariam S.H., Gebreyes W.A., Kefale T.A., Guma G.T, & Berhe N. (2023). Clonal diversity of Staphylococcus aureus isolates in clinical specimens from selected health facilities in Ethiopia. BMC Infectious Diseases, 23, 399.

Publication 2023

StaphType

Clonal Tree

Corresponding Organization : Addis Ababa University

Other organizations : The Ohio State University, Center for Discovery, Hackensack Meridian Health, Ethiopian Public Health Institute

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Variable analysis

independent variables

spa typing was performed using PCR followed by Sanger sequencing

dependent variables

Assignment of spa type Based Upon Repeat Pattern (BURP) analysis for determination of spa clonal complex (spa-CC)
Prediction of the multi-locus sequence types (STs)

control variables

Ridom StaphType was used for BURP analysis
Ridom SpaServer was used to predict the multi-locus sequence types (STs)

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