Cardiac fibroblasts (PDGFR α+) and endothelial cells (CD31 +) were isolated from wild-type C57Bl/6J mouse hearts and sorted using APC-conjugated anti-PDGFR α+ (eBioscience 17-1401-81) and PE-CY7-conjugated anti-CD31 (eBioscience 25-0311-82) antibodies as described previously [37 (link)]. Cardiomyocytes were isolated from wild-type C57Bl/6J mice as described previously [53 (link)].
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